Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free:

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site:, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Google Analytics

Targeted advertising cookies


The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at or by post at:

24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal


Aquapôle INRA

Inra Bordeaux-Aquitaine
Quartier Ibarron
64310 Saint-Pée-sur-Nivelle

tél : +33 (0) 5 59 51 59 51
fax : +33 (0) 5 59 54 51 52

Iban Seiliez


Iban Seiliez
© inra, 2007
Autophagy and fish metabolism

Group « Autophagy » 

Group leader : Iban SEILIEZ, DR2 INRA,, +33 5 59 51 59 99


Team member, 2017

SEITE Sarah, PhD (scholarship : CIFRE),
LESCAT Laury, PhD (scholarship: ED211, UPPA),
LIU Hengtong, PhD (scholarship: China Scholarship Council),
ORY Nicolas, M2,

Groupe Autophagie

 LIU Hengtong ; LESCAT Laury ; DIAS Karine ; SEITE Sarah ; ORY Nicolas ; SEILIEZ Iban

Keywords: Autophagy, Metabolism, Fish, Evolution, Adaptation


Research Topic

The research carried out within our group aims to advance our understanding of the nutritional control of autophagy and its role in the regulation of intermediary metabolism in teleost fish. Due to the wide diversity of their lifestyles, metabolic features and phylogenetic position, these species represent relevant model organisms for studying the metabolic role as well as the evolutionary history of such a key function crucial for adaptation and survival.


Three species are mainly studied:

  • (i) The rainbow trout (Oncorhynchus mykiss): This species displays unusual metabolic features (i.e., a high dietary amino acid requirement and an apparent inability to use dietary carbohydrate for energy furniture) relevant to gain better understanding of the nutritional regulation and the metabolic role of autophagy. Our current work aims to understand the impact of macronutrient composition of the diets (in particular that of the different amino acids) on the formation of autophagosomes. Persons involved in this project: Sarah SEITE, Nicolas ORY, Karine DIAS, Iban SEILIEZ. Collaborators: S PANSERAT (INRA-UPPA, UMR1419 NuMeA, St Pée-sur-Nivelle) ; N CAMOUGRAND et B SALIN  (CNRS, UMR5095 IBGC, Bordeaux).
  • (ii) The European eel (Anguilla anguilla): A critical stage in the life cycle of eel is the estuarine migration to freshwater rearing habitats by the young stages (glass eels). Glass eels do not feed during this stage and rely exclusively on endogenous energy reserves and the establishment of a tightly controlled energy mobilizing system. Numerous studies over the past decade identified Autophagy (a process of cellular self-eating) as a major energy mobilizing system in metazoans. In this context, the present project aims to determine to what extent the estuarine migratory behavior of glass eel may be linked to autophagy activity and evaluate the impact of temperature as well as the existence of genetic polymorphism in the autophagy-related genes, as an assessment of the evolutionary potential of the European eel in face of environmental changes. Persons involved in this project: Hengtong LIU, Karine DIAS, Iban SEILIEZ. Collaborators: V BOLLIET (INRA-UPPA, UMR1224 ECOBIOP, St Pée-sur-Nivelle).
  • (iii) the medaka (Oryzias latipes): This species is now recognized as a relevant model organism and extensively used in many areas of biological research. The work we are doing on this species is part of a project aiming to describe the evolutionary history of autophagy in vertebrates in general and in fish more particularly. Persons involved in this project: Laury LESCAT, Karine DIAS, Iban SEILIEZ. Collaborators: J BOBE and A HERPIN (INRA, UR1037 LPGP, Rennes).

Knowledge gained through such a research program is of great interest from a comparative animal physiological perspective but provide also an entirely new perspective to the issue of the mechanisms involved in tissues construction, metabolism and homeostasis in fish species of high economic, scientific and ecological value.


Cultures primaires

Figure legend: Primary culture of rainbow trout (Oncorhynchus mykiss) myoblasts were incubated in a serum- and amino acid-deprived medium. After 4 h incubation, the immunolocalization of LC3B (green) revealed the appearance of punctate LC3 staining that represents autophagosomes. Nuclei were stained with Hoescht (blue). These results demonstrated for the first time in this species the existence and the control of the autophagosomal system (Seiliez et al., 2012 Autophagy, 8 (3), 1-12).

Representative références

  1. Seiliez, I., Belghit, I., Gao, Y., Skiba-Cassy, S., Dias, K., Cluzeaud, M., Rémond, D., Hafnaoui, N., Salin, B., Camougrand, N., Panserat, S. (2016). Looking at the metabolic consequences of the colchicine-based in vivo autophagic flux assay. Autophagy, 12(2):343-56.
  2. Belghit, I., Skiba-Cassy, S., Geurden, I., Dias, K., Surget, A., Kaushik, S., Panserat, S., Seiliez, I. (2014). Dietary methionine availability affects the main factors involved in muscle protein turnover in rainbow trout (Oncorhynchus mykiss). British Journal of Nutrition, 112 (4), 493-503.
  3. Seiliez, I., Dias, K., Cleveland, B. M. (2014). Contribution of the autophagy-lysosomal and ubiquitin-proteasomal proteolytic systems to total proteolysis in rainbow trout (Oncorhynchus mykiss) myotubes. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 307 (11), R1330-R1337.
  4. Belghit, I., Panserat, S., Sadoul, B., Dias, K., Skiba-Cassy, S., Seiliez, I. (2013). Macronutrient composition of the diet affects the feeding-mediated down regulation of autophagy in muscle of rainbow trout (O. mykiss). Plos One, 8 (9), 12 p.
  5. Seiliez, I., Gabillard, J.-C., Riflade, M., Sadoul, B., Dias, K., Averous, J., Tesseraud, S., Skiba, S., Panserat, S. (2012). Amino acids downregulate the expression of several autophagy-related genes in rainbow trout myoblasts. Autophagy, 8 (3), 1-12.
  6. Seiliez, I., Gutierrez, J., Salmerón, C., Skiba-Cassy, S., Chauvin, C., Dias, K., Kaushik, S., Tesseraud, S., Panserat, S. 2010. An in vivo and in vitro assessment of autophagy-related gene expression in muscle of rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol B Biochem Mol Biol, 157(3):258-66.
  7. Seiliez, I., Gabillard, J.C., Skiba-Cassy, S., Garcia-Serrana, D., Gutierrez, J., Kaushik, S., Panserat, S., Tesseraud, S. 2008. An in vivo and in vitro assessment of TOR signalling cascade in rainbow trout (Oncorhynchus mykiss). Am J Physiol - Regul Integr Comp Physiol, 295: R329–R335.
  8. Seiliez, I., Panserat, S., Skiba-Cassy, S., Fricot, A., Vachot, C., Kaushik, S., Tesseraud, S. 2008. Feeding status regulates the polyubiquitination step of the ubiquitin proteasome-dependent proteolysis in rainbow trout (Oncorhynchus mykiss) Muscle. J Nutr, 138(3): 487-91.