P. viticola genome project

Plasmopara viticola genome project

Plasmopara viticola is an obligatory biotrophic oomycete responsible for grapevine downy mildew, a major and costly grapevine disease. It has been introduced very recently (1878) from North America in Europe and has subsequently invaded vineyards worldwide

At INRA Bordeaux, we carried out a de novo whole-genome sequencing on an isolate (INRA-PV221) of P. viticola, using three Illumina libraries (paired-end, and 3 and 8 kb mate pairs). The assembly was annotated for repeated sequences and gene content (using 454-based RNA-seq data).

GenBank accession : MBPM00000000
Citation : Dussert et al. (2016) Draft Genome Sequence of Plasmopara viticola, the Grapevine Downy Mildew Pathogen. Genome Announcements 4 (5) e00987-16

Dussert et Delmotte

De novo genome sequencing using the PacBio technology is currently under way (june 2016).

To understand how adaptation and demography affected the genetic diversity of European P. viticola populations, we have implemented a population genomics approach by resequencing 50 strains. This data set will allow us to address the following issues :

  • demographic history of the invasion of P. viticola
  • adaptation of the pathogen to cultivated grapevines after its introduction in Europe
  • adaptation to recently released resistant grapevine cultivars
  • molecular bases of pathogenicity

Voir aussi

Funding

ANR GANDALF (ANR-12-ADAP-0009)
ANR EFFECTOORES (ANR-13-ADAP-0003)
European project KBBE InnoVine (ref. 311775)

Collaborations

INRA, UMR441 Laboratoire des Interactions Plantes-Microorganismes, Toulouse (France) site web
INRA, UMR1131 Santé de la Vigne et Qualité du Vin, Colmar (France) site web

Contacts

Yann Dussert (dussert.yann@gmail.com)
François Delmotte (delmotte@bordeaux.inra.fr)

Date de modification : 14 août 2023 | Date de création : 06 juin 2016 | Rédaction : FD, YD