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UR 1264 - MYCSA : Mycologie et securite des aliments

MycSA

Mycologie & Sécurité des Aliments
INRA Bordeaux-Aquitaine
BP 81
33883 Villenave d'Ornon Cedex

Experimental evolution on the plant pathogen fungus, Fusarium graminearum

17 January 2019

Evolution experimentale Fusarium graminearum
© M. Foulongne-Oriol
A new research project at MycSA, funded by the the INRA departement SPE

The Plant Health and Environment Department (SPE) of INRA provides financial support for 2 years for the research project 'EevolFus' leaded by Marie Foulongne-Oriol.
The EevolFus project aims to lay the foundations for a better understanding of the adaptive response of Fusarium graminearum to abiotic stress by studying the involvement  of mutational dynamics in the response to selection pressure throughout the fungus life cycle. This dynamic will be studied by an experimental evolution approach with a strategy Evolve & Resequence .

The selection pressure will be thermal stress to artificially mimic the effects of global warming. The appearance of mutations at the nucleotide level  will be studied on the whole genome by complete resequencing. In order to estimate the effect of successive multiplication cycles on the fitness of the strains, phenotyping of the derived generations will be carried out, for mycelial growth in dishes (by image analysis), the production of mycotoxins (HPLC and mass spectrometry) and aggressiveness on plants (artificial inoculation on wheat).

Expected results are indispensable prerequisites for a better understanding of the underlying molecular mechanisms and evolutionary forces in action.