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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

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Sclerochronology Service

The Sclerochronology Service is organized in different dedicated rooms:

  •     A "wet" room for the reception and dissections of samples as well as for the simplest observations on fish or crustaceans;
  •     A "dry" room for microscopy and more delicate measurements;
  •     A "white" room for the preparation of ultra-clean samples for ultrastructure or microchemistry analysis.
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All rooms can be darkened in order to work on fluorescently marked structures.

Wet area (sorting + prepartion)

It is mainly dedicated to sorting, dissection and short term storage in deep-freezing. We also perform isotope analyses on all tissues.

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Main equipments

  • 10 m of straw mattress
  •     Freezers and a refrigerator
  •     1 Large tank for coarse sorting, rinsing
  •     2 Articulated suction arms (BAO)
  •     1 Fume cupboard; extractor hood
  •     2 Protective masks
  •     1 Eye Wash Shower
  •     1 Chemical cabinet
  •     1 Production of demineralized water
  •     2 Balances, from 5 to 4100 g. d: 0.01g
  •     1 Oven
  •     1 Ultrasonic tank
  •     2 Magnifying lamps
  •     3 Binoculars
  •     1 Cold light generator
  •     2 Colony counters
Esp_humide2

Acquisition area called "dry" room

This is where samples are observed and data are recorded. This space can be a dark room to increase the quality of the observations and especially to visualize epifluorescence marks. A precision balance (0 to 120g. d : 0.1mg) is installed on an anti-vibration table.

Salle_seche_Glob
Imaging stations available:

Microscope Station Nikon 90i
Objectives x 1 x 10 x 20 x 50 x 100 (à sec) ; head, objectives, xyz motorisedFluorescence filters: tetracycline, alizarin and calcein

  •     Intensilight generator for fluorescence; self-centered transmitted light
  •     Polarizer & Analyzer
  •     Digital camera
  •     Dedicated computer with NISD software to make counts and measurements, acquire images and videos. Connection to the internal network and Internet

Binocular Station Nikon SMZ 1500

  • Objectives x 1 and x 0.5, zoom from 0.75 to 11.25Episcopic and diascopic lighting
  • Polarizer & Analyzer
  • Large working distance (110 mm)
  • Trinocular output with digital color camera
  • Dedicated computer with NISD software to make counts and measurements, acquire images and videos. Connection to the internal network and Internet

"White Room"

This laboratory is used for the preparation of clean samples, under controlled atmosphere conditions, for microanalysis, otolith microchemistry, scale and bone treatment. Possible darkness.
The storage room for calcified specimens is used to guarantee the long-term integrity of the collections.

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This laboratory is classified ISO 8.

Main equipments

  • Nikon SMZ 25 acquisition station
    •         Objective 1 and 0.5x
    •         Zoom 0.63 to 15.75
    •         Episcopic and diascopic lighting
    •         Motorized xyz
    •         Fluorescence module (tetracycline, alizarin and calcein)
    •         Large working distance
    •         DSRi2 wide-field camera & dedicated NIS software to acquire large images, mosaic reconstitution, videos, counting and measurement.
  •     Nikon Eclipse Ni Data Acquisition Station
    •         Objectives 10, 20, 50 and 100
    •         xyz motorized turntable
    •         DSRi2 wide field camera & NIS software
    •         Analyzer and polarizer
  •     Laminar flow hood Iso 5
  •     Vacuum oven
  •     1 Polisher Strueurs
  •     Horizontal diamond wire saw Escil W3400
  •     1 ATM Chainsaw Brilliant 221
  •     1 Hot plate up to 450° C
  •     1 Fisher Bioblock Heating Shaker
  •     4 Nalgene drying cabinets
  •     Retsch shredder
  •     Sartorius M-power balance, capacity: 4100 g, d: 0.0.1 g
  •     Eppendorf Centrifuge 5424
  •     Marie Bath LSCI 6L, 5 to 110°C
  •     Vortex mixer Stuart SA8
Salle_blanche2

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