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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal Logo Biogeco Logo Université de Bordeaux

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Le Provost Grégoire

Chercheur

Equipe XylomeS

Grégoire LE PROVOST

UMR 1202 Biodiversité Gènes & Communautés
INRA
69 route d'Arcachon
33610 CESTAS – France

Tel : 05 57 12 27 73
Fax: 05 57 12 28 81

Courriel : gregoire_at_pierroton.inra.fr

Formation

Doctorat, Biologie cellulaire et moléculaire. 2003 Université de Bordeaux I. Effet de la saison et d’un stress mécanique sur la variation du transcriptome dans le xylème en formation chez le pin maritime.
DEA « Biologie forestière ». 1999 Université Henry Poincaré Nancy I. Certification de l’origine génétique et géographique du pin maritime (Pinus Pinaster Ait.) à l’aide de marqueurs microsatellites chloroplastiques.

Thèmes de recherches

Développer des approches de génomique fonctionnelle (transcriptome) pour identifier les gènes impliqués dans la réponse adaptative des arbres forestiers vis-à-vis de contraintes environnementales. Différents modèles utilisés (pin maritime et sécheresse, Chênes blancs européens et anoxie)
Création de bio-puces pin maritime et chênes
Développer des méthodes de microdissection par capture laser afin de se focaliser sur les cellules cibles du caractère étudié (e.g. cellules de garde des stomates pour la sécheresse)
Etude d’expression de gènes candidats en populations naturelles (validation des gènes candidats)
Caractérisation moléculaire de QTLs impliqués dans l’adaptation des arbres forestiers (BSA sur ARNm et puces à ADNc).

Projets de recherches

Bases moléculaires de l’adaptation aux contraintes hydriques chez les arbres forestiers : plasticité et diversité » (projet jeune équipe INRA, C. Plomion coordinateur)
Genomics of wood formation and molecular tools for breeding wood quality in maritime pine. GenoQB (Projet Genoplante, C. Plomion coordinateur)
Apport de la microdissection par capture laser pour les études de génomique fonctionnelle. (Projet innovant INRA, G. Le Provost coordinateur)

Publications récentes choisies

Plomion C, Le Provost G, Pot D, Vendramin GG, Gerber S, Decroocq S, Brach J, Raffin A, Pastuszka P (2001) Pollen contamination in a maritime pine (Pinus pinaster Ait.) polycross seed orchard and certification of improved seeds using cpSSR. Can J For Res 31: 1816-1825

Ribeiro MM*, Le Provost G*, Gerber S, Vendramin GG, Anzidei M, Decroocq S, Marpeau A, Mariette S, Plomion C (2002) Origin identification of maritime pine stands in France using chloroplast single-sequence repeats. Ann For Sci 59 : 53-62

Dubos C, Le Provost G, Pot D, Salin F, Lalane C, Madur D, Frigerio J-M, Plomion C (2003) Identification and characterization of water-deficit responsive genes in hydroponically grown maritime pine (Pinus pinaster Ait.) seedlings. Tree Physiology 23 :169-179

Plomion C, Le Provost G, Stokes A (2001) Wood formation in trees. Plant Physiology 127 : 1513-1523

Le Provost G, Paiva J, Pot D, Brach J, Plomion C (2003). Seasonal variation in transcript accumulation in wood forming tissues of maritime pine (Pinus pinaster Ait.) with emphasis on a cell wall Glycine Rich Protein. Planta 217 : 820-830 (pdf)

Cantón FR*, Le Provost G*, Garcia V, Barré A, Frigério J-M, Paiva J, Fevereiro P, Ávila C, Mouret J-F, de Daruvar A, Cánovas FM, Plomion C (2003) Transcriptome analysis of wood formation in maritime pine. In : Sustainable Forestry, Wood products & Biotechnology, BIOFOR proceeding
Pot D, Mac Millan L, Echt C, Sheree C, le Provost G, Garnier-Géré PH, Plomion C (2005) Nucleotide diversity of genes involved in wood formation in Pinus pinaster and Pinus radiata : footprints of natural selection. New phytologist 167 101-112 (pdf)

Plomion C, Le Provost G, Stokes A (2001) Wood formation in trees. Plant Physiology 127 : 1513-1523
Gion J-M*, Le Provost G*, Lalanne C*, Dumazet H, Plomion C (2003) Mapping of proteins in wood forming tissue of maritime pine using two-dimensional gel electrophoresis and mass spectrometry analysis. Proteomics 5, 3731-3751. auteurs partagés.

Le Provost G, Iskra-Caruana ML, Acina I, Teycheney PY (2006) Improved detection of episomal Banana streak viruses by multiplex immunocapture PCR. Journal of Virological Methods 137 7-13. (pdf)