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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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UMR 1332 Biologie du Fruit et Pathologie

Viral Vectors Platform

Viral Vector Platform

Figure: Use of a viral vector derived from apple latent spherical virus (ALSV) - Upper part: illustration of the extinction of the gene (PDS) involved in the synthesis of carotenoid pigments. VIGS leads to bleaching of the leaves - Lower part: expression of marker genes (fluorochrome GFP or iLOV) via the viral vector.

Viral vectors are a tool of choice to address functional genomics in plants. They allow the transient overexpression or, on the contrary, the specific extinction of a targeted gene in order to understand its function. A viral vector has the advantage of multiplying rapidly in the whole plant with a high level of expression, but it is also easy to manipulate and can be implemented much more rapidly than transgenesis or mutagenesis of plants, especially for plant species that are recalcitrant to transformation.

The technical platform allows us to clone and test viral vectors adapted to the different plant species studied in our research unit. Once optimized, these vectors are able to induce VIGS (Virus induced gene silencing) or gene silencing. In particular, we use cloning methods based on synthetic biology to assemble viral genomes in vitro, and we are developing the use of a viral vector to express RNA-guides in the CRISPR-Cas9 approach.

Contact :

Amandine Bordat :

Sylvie German-Retana :