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Arthur BEAUCHET

Team Flowering, Fruit Development and Environmental Constraints (FDFE)

Wednesday, April 6, 2022- 2 p.m. - Amphitheater Colette & Josy Bové - Campus INRAE Villenave d'Ornon

Abstract :

FW2.2 was identified first as a major QTL controlling for up to 30% of the fruit weight variation. FW2.2 is a negative regulator of cell division but its function remains unclear. Here, gain- and loss-of-function plants and GUS reporter lines under FW2.2 promoter were generated to investigate its mode of action. We demonstrated that FW2.2 is localised to plasmodesmata, a channel that provide direct cytosolic continuity between two cells. Gain-of-function plant show an increase in cell-to-cell diffusion in leaf. Level of callose deposition was measured at two different developmental stages of fruit development and an increase of callose was reported in loss-of-function plant.  On the contrary, level of callose deposition decrease when FW2.2 is overexpressed suggesting a role of FW2.2 in callose deposition/degradation to control cell-to-cell communication.

Jury :

• M. Christian CHEVALIER
  Directeur de Recherche Université de Bordeaux - UMR BFP INRAE Bordeaux - Directeur de Thèse
• Mrs Yoselin ALFONSO-BENITEZ
  Associate Professor University of Leeds - Faculty of Biological Sciences - Rapporteur
• Mrs Esther VAN DER KNAAP
  Professor University of Georgia, Department of Horticulture - Rapporteur
• M. Sébastien MONGRAND
  Directeur de Recherche CNRS - LBM campus INRAE Bordeaux - Examinateur
• M. Jérôme JOUBES
  Directeur de Recherche CNRS - LBM campus INRAE Bordeaux - Examinateur
• Mrs Françoise MONEGER
  Directrice de Recherche ENS de Lyon - Examinateur